FastQCFastQC Report
Sat 18 Jun 2016
SRR3137869_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137869_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4647342
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG290210.624464478835429No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG59970.1290415037240642No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGA51100.10995532500082843No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGT48260.10384430498121293No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT48150.10360761054383344No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCG8900.034.926561
TACCTGG60200.026.8916722
CGACGAG6650.024.90294324
GCTCGAT7150.024.60721864
TACCCGG12850.023.3699382
GTACCTG72600.023.3586731
ACCTGGG62450.023.2037033
CTCGATA8100.022.99890365
TGCGACG7250.022.84225522
ACCCGGG12500.022.633113
TGGTCGT8950.021.20010651
ATGCGAC7650.021.1968921
GCTCGTC9000.021.08255439
CGTACGA3200.020.48339860
ATCGTCA7950.020.39525410
ACGAGTC8600.020.05869326
TGCGCGA4600.019.49969912
GTACTTT74900.019.43824414
GAGTACT78900.019.1955212
AGTACTT79050.018.94129213
Produced by FastQC (version 0.11.5)