FastQCFastQC Report
Sat 18 Jun 2016
SRR3137869_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137869_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4647342
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG116680.2510682450312458RNA PCR Primer, Index 39 (96% over 30bp)
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG64880.13960668270163892No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGT53870.1159157212875661No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGA52060.11202102190886747No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT51760.11137549162510527No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG50010.10760989830315909RNA PCR Primer, Index 39 (96% over 30bp)
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTAC48610.10459742364560215No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG29650.032.928443
ATCTCGT31800.030.81059537
CTCGTAT31950.030.77392439
GTACCCG8250.030.5324671
TCGTATG33500.029.76198840
CGACGAG8850.029.23659924
TCTCGTA33900.029.10550938
GCTCGAT7550.028.78771464
TGCGACG9000.028.74932322
ATCGTCA9550.028.5392110
ATGCCGT34500.028.29933444
TGCCGTC35600.027.32800945
CCGTCTT36000.027.12048747
TACCTGG61850.026.779012
CATCTCG38950.025.8633236
CGTATGC38700.025.4063841
CGTCTTC39050.025.09058848
ACGAGTC10500.024.64227926
ATGCGAC10800.024.59664221
GCCGTCT39700.024.4188246