Basic Statistics
Measure | Value |
---|---|
Filename | SRR3137869_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 4647342 |
Sequences flagged as poor quality | 0 |
Sequence length | 75 |
%GC | 44 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG | 11668 | 0.2510682450312458 | RNA PCR Primer, Index 39 (96% over 30bp) |
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG | 6488 | 0.13960668270163892 | No Hit |
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGT | 5387 | 0.1159157212875661 | No Hit |
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGA | 5206 | 0.11202102190886747 | No Hit |
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT | 5176 | 0.11137549162510527 | No Hit |
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG | 5001 | 0.10760989830315909 | RNA PCR Primer, Index 39 (96% over 30bp) |
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTAC | 4861 | 0.10459742364560215 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TATGCCG | 2965 | 0.0 | 32.9284 | 43 |
ATCTCGT | 3180 | 0.0 | 30.810595 | 37 |
CTCGTAT | 3195 | 0.0 | 30.773924 | 39 |
GTACCCG | 825 | 0.0 | 30.532467 | 1 |
TCGTATG | 3350 | 0.0 | 29.761988 | 40 |
CGACGAG | 885 | 0.0 | 29.236599 | 24 |
TCTCGTA | 3390 | 0.0 | 29.105509 | 38 |
GCTCGAT | 755 | 0.0 | 28.787714 | 64 |
TGCGACG | 900 | 0.0 | 28.749323 | 22 |
ATCGTCA | 955 | 0.0 | 28.53921 | 10 |
ATGCCGT | 3450 | 0.0 | 28.299334 | 44 |
TGCCGTC | 3560 | 0.0 | 27.328009 | 45 |
CCGTCTT | 3600 | 0.0 | 27.120487 | 47 |
TACCTGG | 6185 | 0.0 | 26.77901 | 2 |
CATCTCG | 3895 | 0.0 | 25.86332 | 36 |
CGTATGC | 3870 | 0.0 | 25.40638 | 41 |
CGTCTTC | 3905 | 0.0 | 25.090588 | 48 |
ACGAGTC | 1050 | 0.0 | 24.642279 | 26 |
ATGCGAC | 1080 | 0.0 | 24.596642 | 21 |
GCCGTCT | 3970 | 0.0 | 24.41882 | 46 |