FastQCFastQC Report
Sat 18 Jun 2016
SRR3137868_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137868_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3975576
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG340700.8569827365896163No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG56070.14103616683469264No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGT44820.11273838055164837No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACTGCAAATTCTAGAATTGTGAGTAGAA43100.10841196344881848No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGA41810.10516715062169608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCG6300.027.41471
ATGCGAC5250.025.6311121
CGTACGT700.001334533724.64250634
TACCCGG7050.024.4794392
TACCTGG47000.023.6471392
ACGACCG1554.0961822E-822.25634869
ATCGTCA5100.021.64566210
TGGTCGT5400.021.08170951
TGTTTCG7500.020.69840466
TGCGACG5800.020.2261422
GCTCGAT5300.020.17768964
ATTTAGG28550.020.0842671
ACCTGGG45650.020.0293663
CGACGAG6050.019.96015224
GTCGTCT5400.019.8040353
GAGTACT68500.019.74328212
TAAATCG6150.019.63263162
CTATTGA27850.019.5712039
GCTATTG28400.019.435128
GATACGA4800.019.40475548
Produced by FastQC (version 0.11.5)