FastQCFastQC Report
Sat 18 Jun 2016
SRR3137866_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137866_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4264963
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG180260.4226531390776426No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG54370.1274805901012506No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGA47930.11238081080656502No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGT46510.10905135636581138No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT44280.10382270608209263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG5400.033.86463524
TACCTGG60300.032.456622
GTACCCG6450.030.5241181
GCTCGAT6250.029.80516464
ACCTGGG60600.029.379643
GTACCTG71250.027.8262711
ATCGTCA6350.026.62013810
CCTGGGG44800.026.5661774
ATGCGAC7300.025.99616421
TGCGACG7350.024.41070422
CGTCATT8350.024.37921326
TGGTCGT7600.024.05720351
GTACGTG6200.023.9554731
GTCGTCT7800.023.88262253
GTCAGCG8400.023.81916243
TACGTGG6250.023.7479422
GCTCGTC8350.023.13633739
GCGCTGG8350.023.13552547
CTGGGGG34700.022.865265
TAGGGCG5050.021.86212531
Produced by FastQC (version 0.11.5)