SRR3137866_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3137866_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4264963
Sequences flagged as poor quality	0
Sequence length	75
%GC	44

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG	6972	0.16347152366855236	RNA PCR Primer, Index 27 (96% over 30bp)
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG	5684	0.1332719650791812	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGT	5232	0.12267398333819074	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT	4802	0.11259183256689448	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGA	4779	0.11205255473494143	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTAC	4669	0.10947339988647029	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
ATCGTCA	640	33.422108	10
TATGCCG	1915	33.32843	43
TACCTGG	6185	32.858566	2
GCTCGAT	680	32.47005	64
CTCGTAT	1990	31.89897	39
TCTCGTA	2140	29.501852	38
ACCTGGG	6345	29.039066	3
TGCGACG	760	28.598215	22
TCGTATG	2255	28.456295	40
CTCGATA	780	28.307228	65
GTACCTG	7255	28.250233	1
CCGTCTT	2305	27.83902	47
ATGCGAC	815	27.514883	21
TATCTCG	2280	27.387709	36
GCTCGTC	795	27.33917	39
CCTGGGG	4775	26.591785	4
TGGTCGT	805	26.57099	51
TGCCGTC	2430	26.406973	45
ATGCCGT	2450	26.332218	44
GTCGTCT	830	25.770658	53