FastQCFastQC Report
Sat 18 Jun 2016
SRR3137864_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137864_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2860024
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG198440.6938403314098064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCG6500.032.946971
AGTAGGC20200.030.5889992
GACGTAC8500.030.43755169
AGTACGC11700.029.7988222
GTACGCA12050.029.20753
GACTAAC7050.027.89029947
CGCAGTG13400.026.772936
TACCTGG35700.026.6872862
GTAGGCA24550.026.2827843
TAGGCAG23500.026.2778914
CGACGAG2400.025.87825224
TACGCAG13500.025.8102934
ACTAACC11850.025.61720348
TACCCGG7750.025.3884832
GAGTAGG26400.025.2516981
GTACACG5300.024.7653751
GAGTACG15400.024.6722741
GTACCTG40400.024.537921
TATGCCG1701.6734703E-1024.3500450
ACCCGGG7350.023.9422043
Produced by FastQC (version 0.11.5)