FastQCFastQC Report
Sat 18 Jun 2016
SRR3137864_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137864_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2860024
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG68940.24104692827752494RNA PCR Primer, Index 46 (96% over 28bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA41300.1444043826205654No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG31690.11080326598657912RNA PCR Primer, Index 46 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTAA4650.034.12901769
TATGCCG21200.034.0111843
GCGAATC21800.032.2838233
CTCGTAT21400.032.081239
GTACCCG8100.031.947781
ATCTCGT21600.031.78415137
CGAATCT22200.031.5467334
TCGTATG23100.031.06437740
TGCCGTC22900.031.03437645
TCTCGTA22150.030.83917438
ATGCCGT23750.030.50471344
CGTATGC23900.030.31326541
AATCTCG22750.030.02583336
AGTAGGC21650.029.6428182
CCGTCTT24100.029.0601547
GGCGTAC21250.028.8988169
CGTCTTC23900.028.8702848
GTATGCC26450.027.2603842
GAATCTC25750.027.19756735
AGGCGTA20700.026.99998568
Produced by FastQC (version 0.11.5)