FastQCFastQC Report
Sat 18 Jun 2016
SRR3137863_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137863_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences631958
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG465847.371375945869821No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGTC200.0073583151.74857317
CCGCGCA200.0073583151.74857337
CGATACT200.0073583151.74857347
TTCCTCG200.0073583151.74857355
AGTAGGC2250.036.8164672
GTACCCG1700.036.5747071
AACGACG852.9649527E-936.52840865
CCGTATC1151.8189894E-1235.99901252
CGAATTC1151.8189894E-1235.99901252
GGTCGCC1201.8189894E-1234.4990547
CGGCAAA501.8607744E-434.4990567
CGATTAC905.18412E-934.4990558
GTAGGCA2650.033.8534853
CGCCGTA1253.6379788E-1233.11908750
GAGTAGG2850.032.724741
TACCCGG1950.031.8604012
TCGCCGT1305.456968E-1231.84527849
GTGGTCG1305.456968E-1231.84527845
GCCGTAT1305.456968E-1231.84527851
TGGTCGC1305.456968E-1231.84527846
Produced by FastQC (version 0.11.5)