SRR3137863_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3137863_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	631958
Sequences flagged as poor quality	0
Sequence length	75
%GC	46

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG	14270	2.2580614534510204	RNA PCR Primer, Index 46 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG	8727	1.380946202121027	RNA PCR Primer, Index 46 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG	6105	0.9660452118653455	RNA PCR Primer, Index 46 (96% over 28bp)
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGG	1646	0.2604603470483798	TruSeq Adapter, Index 4 (96% over 27bp)
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGG	1513	0.2394146446441061	TruSeq Adapter, Index 4 (96% over 27bp)
TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGG	945	0.1495352539251026	RNA PCR Primer, Index 46 (96% over 28bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	916	0.1449463413707873	No Hit
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAG	894	0.14146509736406532	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	733	0.11598872076941823	No Hit

Adapter graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGCA	20	8.505134E-5	69.00997	4
ACGATTC	35	3.285095E-7	59.142048	38
TACGATT	35	3.285095E-7	59.142048	37
CTCGATA	50	6.9394446E-8	48.299335	65
TATCACG	30	6.304795E-4	46.00665	2
ATCACGC	30	6.304795E-4	46.00665	3
CGATTCG	30	6.3097145E-4	45.999367	39
TCGTCAG	55	1.4685247E-7	43.908485	41
TGGTCGT	55	1.4685247E-7	43.908485	51
CTGGTCG	65	1.1370503E-8	42.460953	50
TCGTCTA	60	2.9081093E-7	40.249447	54
GCTCGAT	60	2.9081093E-7	40.249447	64
GGCGTAA	45	1.0011349E-4	38.33281	69
GATAGTG	55	7.3898173E-6	37.635845	7
CGTCTAC	65	5.4466364E-7	37.153336	55
TGCCGTA	65	5.4466364E-7	37.153336	45
AGATAGT	75	4.0106897E-8	36.805317	6
GTCGCAT	50	1.8606235E-4	34.499527	22
CTCGTCA	70	9.728064E-7	34.499527	40
CGCTGGT	70	9.728064E-7	34.499527	48