FastQCFastQC Report
Sat 18 Jun 2016
SRR3137862_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137862_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3321076
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG265680.7999816926803242No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31000.042.1116071
GTATCAA67550.035.8981171
ATCAACG88000.027.3647123
TCAACGC89300.027.0878014
CAACGCA91200.027.0183145
TATCACG2700.025.514322
AACGCAG98200.025.2022116
TATCAAC98250.024.260032
TACCTGG20550.023.13052
CGCAGAG111350.021.7082048
ACGCAGA117350.020.9211947
GTCGTAT4300.020.85668427
ACATGGG93250.020.7996443
GTACATG99750.020.7532391
GTACCTG23350.020.6521591
TAACGCA3700.020.4884474
TACATGG99400.020.1676062
AGAGTAC116400.019.64365211
GAGTACT81700.019.33355312
ATAACGC3400.019.251793
Produced by FastQC (version 0.11.5)