FastQCFastQC Report
Sat 18 Jun 2016
SRR3137862_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137862_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3321076
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG40410.12167743225388397RNA PCR Primer, Index 46 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33850.042.1025471
CTCGTAT17950.034.40440439
TATGCCG17000.034.29807743
AATCTCG20350.034.24465636
GTATCAA70100.034.2124181
TCTCGTA18400.033.5619838
GCGAATC25850.033.0991333
ATAACGC2450.032.3887183
TCGTATG18650.032.00314740
CGAATCT22900.031.63801634
ATGCCGT17650.031.47118844
CGAATCG4100.031.13460434
ATCTCGT21750.030.77193837
AATCGCG3400.030.44024736
TGCCGTC18700.030.25758245
GGCGAAT31500.029.90043632
CCGTCTT20000.029.15336647
CGTATGC21550.028.01707341
ATCAACG86250.027.320813
TCAACGC87600.027.1360784
Produced by FastQC (version 0.11.5)