FastQCFastQC Report
Sat 18 Jun 2016
SRR3137861_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137861_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4620461
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG313940.6794560109911111No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48900.041.073951
GTATCAA95550.032.1979061
CTCGATA6800.026.39380565
CAACGCA120150.025.6464755
TCAACGC118600.025.5116754
ATCAACG119100.025.3712393
TATCAAC120500.025.0729392
GCTCGAT7300.024.58547664
AACGCAG134700.023.1365996
CGCAGAG147550.020.658848
ATGCGAC6400.020.47881721
CGTCATT10000.020.3556626
CCGATAA18150.020.34259438
GTACATG149500.020.0255931
ACGCAGA154000.019.9494537
GTGGTAT22400.019.8403111
CGCACTA5950.019.71941858
CATACCG19100.019.6914734
ACCGATA19000.019.6132937
TACCGAT18450.019.44989436
Produced by FastQC (version 0.11.5)