FastQCFastQC Report
Sat 18 Jun 2016
SRR3137861_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137861_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4620461
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44700.042.0778051
GTATCAA91300.033.9067151
TCAACGC116300.026.1932834
CGCACTA5850.025.94923658
ATCAACG117150.025.9443343
TATCAAC118550.025.928962
CAACGCA119000.025.569995
CTCGATA6950.024.82090865
ATGCGAC7150.023.15979821
AACGCAG134650.022.7766726
GCTCGAT8050.021.4292364
TACCTGG23850.021.4083042
TATGCCG8900.021.32018943
AGCATCG28150.021.20267934
TCGGGTA3300.020.90973538
CGCAGAG147300.020.3285858
AATGCGA7500.020.2388720
CAATGCG7500.020.2388719
TAGGTCG9100.020.092521
ATCGGGG11200.020.02293837
Produced by FastQC (version 0.11.5)