FastQCFastQC Report
Sat 18 Jun 2016
SRR3137859_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137859_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3997835
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG239420.5988741406286152No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48250.041.8335041
GTATCAA105900.033.501571
CGACGAG7300.029.7681224
TGGTCGT8350.029.76132451
GCTCGAT8800.028.63416964
ATCAACG125500.027.9721053
CAACGCA129400.027.5646925
TCAACGC129400.027.1870654
TATCAAC130950.026.828532
GCTCGTC10150.026.51941339
ATGCGAC9100.026.15223121
AACGCAG140850.025.4255946
GTCGTCT10000.024.85039553
CTCGATA10100.024.60743965
ATCGTCA8950.023.88485510
CAATGCG9250.023.8643819
GTACATG147000.023.431851
CGTACGA5350.023.22525260
AATGCGA10100.023.22228420
CTGGTCG11800.022.81348250
Produced by FastQC (version 0.11.5)