FastQCFastQC Report
Sat 18 Jun 2016
SRR3137859_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137859_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3997835
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG67550.16896645309273645TruSeq Adapter, Index 11 (96% over 28bp)
GTCCTATGCTGTCCTCTGGGTGCCTCTGTTCTAGCTGTCACTACTGTGATCAGCTGGTCTCCCTCGACACGTGGT41100.1028056435545739No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47750.043.6590041
TATGCCG24300.035.06842443
CTCGTAT23500.034.3541339
TCTCGTA23500.034.353738
GCTCGAT9200.032.99925664
GTATCAA104400.032.4323771
CCGTCTT26200.032.39361247
TGGTCGT9150.031.672651
ATGCCGT27350.031.0315444
TCGTATG27250.031.01958540
ATCTCGT26200.030.94511637
GATCTCG26200.030.81304736
CTCGATA10150.030.59043965
TGCCGTC28200.030.09619145
GCCGTCT31150.027.68901446
CTAGATC30900.026.57287833
CGTCTTC32200.026.46434648
TCGATAA11600.026.1721566
CGTCATT11600.026.17182226
GTCGTCT11600.025.87538553
Produced by FastQC (version 0.11.5)