FastQCFastQC Report
Sat 18 Jun 2016
SRR3137858_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137858_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3591028
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG294610.8204057445388897No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39300.041.1080061
GTATCAA83600.034.570191
ATCAACG106150.027.3285713
TCAACGC107000.027.1808034
CAACGCA110650.026.695755
AACGCAG120150.024.7882046
TATCAAC117500.024.4204252
CGTACGA1953.8198777E-1123.00802460
CGCAGAG134500.021.8658688
TAACGCA3950.021.809544
TACCTGG21250.021.5595052
ATAACGC3850.021.4770873
GTACATG118000.021.2225971
GTACCTG22800.021.0003261
ACGCAGA143200.020.6334447
ATGCGAC3550.020.40302821
TACATGG119450.020.2728712
AGAGTAC137800.020.1229811
ACATGGG113350.020.1215443
CAGAGTA143150.019.75254610
Produced by FastQC (version 0.11.5)