FastQCFastQC Report
Sat 18 Jun 2016
SRR3137858_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137858_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3591028
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG42870.11938085695795188RNA PCR Primer, Index 46 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38100.045.6574021
GTATCAA83850.036.058421
AATCGCG4200.032.85661336
TCTCGTA18500.031.88867638
CTCGTAT18500.031.8882339
TATGCCG19600.031.85962543
CGAATCG5450.031.6530734
GCGAATC28550.030.81643733
AATCTCG21900.030.08855636
ATGCCGT20250.029.30363844
TCGTATG20150.028.93501540
CGAATCT24650.028.55325134
ATCTCGT23600.027.48260737
GGCGAAT35250.027.40610332
TGCCGTC20850.027.30247945
TCAACGC108100.027.2879334
ATCAACG108100.027.2563973
CAACGCA109050.027.1451245
CGCACTA3700.027.04048258
CCGTCTT21800.026.11232847
Produced by FastQC (version 0.11.5)