FastQCFastQC Report
Sat 18 Jun 2016
SRR3137856_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137856_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4121836
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG369530.8965179594724293No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41600.040.82461
GTATCAA87250.033.9548151
CGCGTAA500.0077180127.61404666
ATCAACG105750.027.5640343
TCAACGC107000.027.2137934
CAACGCA110050.027.0591265
TATCAAC108900.026.6023862
AACGCAG116600.025.8985166
ACGCAGA131400.022.6945577
ATAACGC3500.022.6418863
CGCAGAG134350.021.96698
GTACATG107350.020.9866921
TGCGACG3150.020.80592722
GAGTACT94500.020.65313512
TACATGG105000.020.6026382
AGAGTAC138000.020.4397511
TACCTGG24800.020.1404342
CAGAGTA144600.019.7210
ACATGGG108500.019.656843
AGTACTT99800.019.41906213
Produced by FastQC (version 0.11.5)