FastQCFastQC Report
Sat 18 Jun 2016
SRR3137856_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137856_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4121836
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG100410.24360503426143104TruSeq Adapter, Index 1 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG49930.12113533871798879TruSeq Adapter, Index 1 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43050.038.8823621
CTCGTAT34650.033.8529439
TATGCCG37600.033.3998743
TCTCGTA35650.032.90294638
TCGTATG38050.032.82312440
GTATCAA85200.032.1231161
ATGCCGT39650.031.58600444
ATCTCGT37200.031.16102837
CCGTCTT40550.031.05436747
TGCCGTC40850.030.65813845
GCCGTCT41650.030.31740846
GATCTCG38300.030.17598336
CGTCTTC43500.028.86942148
CGTATGC44000.028.38488441
ATCAACG101950.026.1942563
TGCGACG2800.025.87415322
TCAACGC103250.025.8304044
TATCAAC105150.025.6274032
GTATGCC49100.025.5770942
CAACGCA104550.025.5752245
Produced by FastQC (version 0.11.5)