FastQCFastQC Report
Sat 18 Jun 2016
SRR3137855_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137855_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4847009
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG275540.568474290020918No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55200.038.559041
GTATCAA112500.034.502311
ATCAACG137600.027.7644183
CAACGCA142150.027.3469095
TCAACGC140000.027.294664
ATGCGAC7400.027.03317621
TATCAAC147950.025.8403422
CGCACTA7500.025.76684258
AACGCAG155400.025.1075486
AATGCGA8250.024.2481920
CTCGATA7950.023.88118665
GCTCGAT8000.023.73143664
CAATGCG8650.023.52562519
ATCGTCA9200.023.23837910
CGACGAG8700.022.99611324
CGCAGAG169000.022.543628
TGGTCGT8800.021.96333351
ACGCAGA176250.021.7721167
GTACTAG15500.021.5534521
GTCGTCT9200.021.0079753
Produced by FastQC (version 0.11.5)