FastQCFastQC Report
Sat 18 Jun 2016
SRR3137855_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137855_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4847009
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG73860.15238263432149599RNA PCR Primer, Index 27 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58100.040.510931
GTATCAA112350.033.54391
TATGCCG26150.031.92745243
CTCGTAT26000.030.5190239
TCTCGTA26400.030.31828538
ATCTCGT27650.028.94765937
ATGCCGT29650.028.04225544
TCGTATG29200.027.8833740
TATCTCG28650.027.81684936
TGCCGTC29150.027.69534945
CCGTCTT29800.027.20703147
ATCAACG138450.026.5152763
AATGCGA7950.026.47015220
TCAACGC140050.026.2859884
CAACGCA141250.026.135955
GCCGTCT31600.025.43890446
TCGACGA3800.025.42166351
CGACGAG8300.025.35472524
TATCAAC150050.024.8336012
ATGCGAC8400.024.64141821
Produced by FastQC (version 0.11.5)