FastQCFastQC Report
Sat 18 Jun 2016
SRR3137853_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137853_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6574381
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG435030.6617048814177334No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68050.039.1265451
GTATCAA136300.033.1556931
TGGTCGT14350.028.38034651
CTCGATA15450.026.14054165
ATCAACG172000.025.5584773
CAACGCA174300.025.5062945
TCAACGC174400.025.2880844
GTACTAG19400.025.034421
GCTCGAT15900.024.74884864
TATCAAC183200.024.2719332
AACGCAG188700.023.8560836
ATGCGAC15850.023.503221
CGTCATT17800.023.06405426
GTACATG239050.022.6221
GTCGTCT18050.022.17916753
CGTACGA8450.022.05437960
ATCGTCA15800.022.04166610
CAATGCG16000.021.98886319
AATGCGA16450.021.59784520
ACATGGG235650.021.5351563
Produced by FastQC (version 0.11.5)