FastQCFastQC Report
Sat 18 Jun 2016
SRR3137853_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137853_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6574381
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG101130.15382436764769183RNA PCR Primer, Index 17 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67100.040.3772241
TATGCCG40100.036.22139443
CTCGTAT34000.035.10896339
AATCTCG36200.034.4045736
TCTCGTA35300.034.10920338
GTATCAA139000.033.8182871
ATCTCGT37150.033.2464337
TCGTATG42800.033.12980340
ATGCCGT46250.031.55411744
CCGTCTT46400.030.41137547
TGCCGTC47200.030.18830945
CGTATGC51350.028.2184441
GCCGTCT52050.026.97766746
CAACGCA172850.026.687185
ATCAACG172550.026.593613
GTACTAG19600.026.4134121
TCAACGC175950.026.2756144
ATCGTCA15300.026.15477810
GCTCGAT17150.026.15190764
TGGTCGT17850.025.89985551
Produced by FastQC (version 0.11.5)