FastQCFastQC Report
Sat 18 Jun 2016
SRR3137850_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137850_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3826940
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG246060.6429680109957303No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43200.043.4530871
GTATCAA93000.033.3324661
TCAACGC119300.025.5306434
ATCAACG120000.025.4324843
CAACGCA122900.025.3755175
TACCTGG20300.024.767022
TATCAAC125500.024.1740322
AACGCAG132550.023.6627456
GTACATG150050.022.8169841
ACATGGG151100.021.7480433
ACCTGGG24050.021.3406413
ATGCGAC4400.021.16406221
CGTACGA2451.8189894E-1221.12908460
GTACCTG24650.021.0992931
TACATGG161450.021.0094722
CTCGATA5150.020.77705665
CATGGGG80900.020.2299614
CGCAGAG149850.020.2014928
ACGCAGA154050.020.0075117
ACTAGCG3050.019.23580255
Produced by FastQC (version 0.11.5)