FastQCFastQC Report
Sat 18 Jun 2016
SRR3137850_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137850_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3826940
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG70850.185134859705143RNA PCR Primer, Index 39 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44950.046.150331
GTATCAA96750.034.570271
TATGCCG24000.034.50007243
CTCGTAT24050.034.14188839
TCTCGTA24000.033.7817638
ATCTCGT24900.032.42217637
ATGCCGT26350.031.68507644
TGCCGTC27200.030.4412445
CCGTCTT27300.030.20335847
TCGTATG28350.029.69357140
TACCTGG21700.027.823732
GCCGTCT30450.027.41877646
TCAACGC118150.027.2733734
CAACGCA119300.027.010475
GCTCGAT4600.026.99970464
ATCAACG120550.026.759013
ATGCGAC4700.025.69120821
TATCACG2150.025.6754782
TATCAAC128200.025.4320162
AATGCGA4500.025.2990620
Produced by FastQC (version 0.11.5)