FastQCFastQC Report
Sat 18 Jun 2016
SRR3137849_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137849_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3910116
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG304910.7797978371997148No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43600.041.9421271
GTATCAA92100.033.391371
ATCAACG116600.026.1727813
TCAACGC117850.025.957624
TATCAAC118600.025.6080362
CAACGCA122750.025.5711775
GCTCGAT4850.024.91208664
AACGCAG130650.024.1090266
TGGTATC23150.022.1629522
CTCGATA5250.021.69977265
TAACGCA3850.021.4749414
CGCAGAG145450.021.2846728
ACGCAGA147600.021.1373167
GTGGTAT25600.020.9858721
ATGCGAC5300.020.82577721
TACCTGG22250.020.7380122
GTACATG128600.020.3791521
AGAGTAC151050.019.61072711
TACATGG131600.019.5197982
TGGTCGT6200.019.4854351
Produced by FastQC (version 0.11.5)