FastQCFastQC Report
Sat 18 Jun 2016
SRR3137849_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137849_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3910116
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG88970.2275380065450744RNA PCR Primer, Index 39 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG41820.10695334869860638RNA PCR Primer, Index 39 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45250.040.890041
GTATCAA91350.032.64951
CTCGTAT27300.032.2250339
TATGCCG28200.031.93060943
TCTCGTA28250.030.8971138
ATGCCGT30200.029.81599844
ATCTCGT29600.029.48757437
TCGTATG30800.029.45919240
CCGTCTT30700.029.4420247
TGCCGTC30950.029.42713245
GCCGTCT31550.028.64881346
GCTCGAT5000.028.28978564
ATCAACG108150.026.8638363
CATCTCG33150.026.74606136
TCAACGC109650.026.5274724
CAACGCA111800.025.9864645
TATCAAC114500.025.7366182
TGGTCGT5550.025.4866251
TACCTGG23700.025.1881832
TAACGCA3400.024.3561844
Produced by FastQC (version 0.11.5)