FastQCFastQC Report
Sat 18 Jun 2016
SRR3137847_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137847_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4422953
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG370270.8371556288298789No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40050.040.408561
GTATCAA81050.035.7714961
TCAACGC105050.027.5475274
CAACGCA107000.027.245775
ATCAACG106650.027.0979543
TATCAAC113350.025.2445352
AACGCAG118400.024.7427866
GTCGTAT7500.024.37947727
GTACATG140450.021.3537651
CGCAGAG135600.021.2058228
TGTCGTA8050.020.99954826
ATAACGG13500.020.45305363
CCGATAA12150.020.45026838
ACGCAGA141850.020.3684737
ACATGGG136950.020.121643
TACATGG146950.020.011342
ACCGATA12800.019.67982137
GTGGTAT18750.019.649911
AGAGTAC139850.019.48014311
GATAACG13900.019.36808462
Produced by FastQC (version 0.11.5)