FastQCFastQC Report
Sat 18 Jun 2016
SRR3137847_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137847_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4422953
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG98850.2234932182186878TruSeq Adapter, Index 1 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG49210.11126050853355213TruSeq Adapter, Index 1 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39850.040.7067261
GTATCAA81400.034.6837121
TATGCCG36350.033.4097243
TCGTATG37050.032.498440
CGTATGC37300.032.46557241
CTCGTAT34450.032.24715839
TCTCGTA34550.031.8535338
ATCTCGT35500.031.09829537
TGCCGTC40900.029.94605845
GATCTCG37050.029.70417236
ATGCCGT41300.029.48894544
CCGTCTT42450.028.52752147
GCCGTCT44750.027.138446
CAACGCA104800.026.7321725
TCAACGC104500.026.6438374
ATCAACG104650.026.6056463
CTCGATA4350.025.37878265
CGTCTTC47750.025.28857448
TATCAAC112400.025.0474412
AACGCAG116100.024.1886736
Produced by FastQC (version 0.11.5)