FastQCFastQC Report
Sat 18 Jun 2016
SRR3137845_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137845_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5099888
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG384750.7544283325437735No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54100.040.940741
GTATCAA110350.033.8999821
GCTCGAT7900.031.4576864
CTCGATA9050.027.0799765
ATCAACG137800.026.429393
CAACGCA141250.026.2101335
TCAACGC139750.025.9917494
TGGTCGT10100.025.62922951
TATCAAC144800.025.4528182
GCTCGTC11000.024.46925239
CAATGCG8250.024.24842519
AACGCAG154600.024.1278216
ATGCGAC8350.023.95849621
CGTCATT11250.023.30376426
GTCGTCT11350.021.89371553
ATCGTCA9150.021.48236710
TTGCGCC12600.021.35763431
AATGCGA9550.021.30938520
CGCAGAG174850.020.6879238
GTCGTCA12900.020.58924524
Produced by FastQC (version 0.11.5)