FastQCFastQC Report
Sat 18 Jun 2016
SRR3137845_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137845_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5099888
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51000.041.0137671
GTATCAA105400.033.9598161
GCTCGAT8850.030.4061464
CTCGATA8900.030.23561765
ATCAACG129800.026.7935263
TCAACGC130550.026.7186244
CAACGCA130850.026.6309975
ATCGGGG11650.026.35618437
AGCATCG30300.026.30194334
CAATGCG8300.026.18516719
GTCGTCT10400.025.87547153
TGGTCGT10200.025.70660251
TATCAAC139450.025.2118072
GCTCGTC10700.025.14974439
AACGCAG144200.024.2851356
TATGCCG9850.023.81722543
ATGCGAC9300.023.37001821
ATCGTCA9300.023.3695610
CGCGGAT3200.022.64081639
CATCGGG20950.022.3961536
Produced by FastQC (version 0.11.5)