FastQCFastQC Report
Sat 18 Jun 2016
SRR3137844_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137844_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4986689
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG288610.5787607769403706No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG9050.037.7352824
GGTATCA52050.037.724331
TGCGACG9450.035.40510622
CAATGCG10150.033.30052619
GTATCAA102250.032.511081
ATGCGAC11700.030.06836121
ATCGTCA11550.028.96053710
CGTACGA5900.028.07561160
GCGACGA12200.027.70745323
AATGCGA12900.027.53922520
ACGAGTC12500.026.77056926
CGCACTA12300.026.37213558
GCTCGAT11300.026.26817764
ATCAACG125250.026.0791823
CGGTCGA2250.026.05845813
CAACGCA129350.025.822065
GTACTAG15900.025.7820361
CTCGATA11650.025.77630865
TCAACGC130400.025.0791654
TATCAAC131950.024.7480032
Produced by FastQC (version 0.11.5)