FastQCFastQC Report
Sat 18 Jun 2016
SRR3137844_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137844_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4986689
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG64950.13024674287889218RNA PCR Primer, Index 17 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52200.037.4944571
CGACGAG9400.035.2340624
CAATGCG10000.033.1173619
TATGCCG26600.033.07332643
TGCGACG10150.032.62957822
GTATCAA105200.031.7624591
AATCTCG22400.030.80358536
CTCGTAT22300.030.32288439
CTCGATA11000.030.10850165
TCGATAA11300.029.61505566
ATGCGAC12050.029.48875221
TCGTATG29650.028.97361440
GCTCGAT11500.028.4994464
CCGTCTT31250.028.04133247
AATGCGA12200.027.99366620
ATGCCGT31200.027.86539644
TCTCGTA24650.027.71197938
TGCCGTC32600.027.30369445
GCCGTCT32050.026.91108746
ATCAACG122800.026.1858883
Produced by FastQC (version 0.11.5)