FastQCFastQC Report
Sat 18 Jun 2016
SRR3137843_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137843_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5196681
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG431630.8305878309636477No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG77880.14986488491404418No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGC61390.11813309302610647No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76750.041.3697171
CGACGAG15800.036.6763324
CAATGCG16050.035.88598319
GTATCAA160800.034.7586171
GCTCGAT17300.034.71807564
CTCGATA17450.033.82782765
ATCGTCA17500.033.29934710
TGCGACG17900.032.37352422
TGGTCGT19300.032.18879351
GTCGTCT20450.030.37866853
ATGCGAC20500.029.44287521
GTACTAG23000.029.0471741
CGATAAT20650.028.5851967
ATCAACG200100.027.3007133
GCTCGTC22900.027.12645139
CAACGCA204800.027.074585
TCGATAA22750.027.00843666
TATCAAC208050.026.6331482
TCAACGC209350.026.2483444
TCGTCTA24650.026.18268654
Produced by FastQC (version 0.11.5)