FastQCFastQC Report
Sat 18 Jun 2016
SRR3137843_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137843_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5196681
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG122900.23649710266995413TruSeq Adapter, Index 11 (96% over 28bp)
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG91880.1768051569838518No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGC69460.13366223556920273No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76300.040.624711
CGACGAG14100.039.14761424
GCTCGAT20200.037.57443664
CAATGCG14750.037.42102419
CTCGATA20350.036.9580565
CTCGTAT41300.035.9199239
TATGCCG42850.035.5874443
ATCGTCA15750.035.26445810
TGGTCGT21850.034.8959151
TCGTATG44700.034.2682940
GTCGTCT22650.033.96803353
TCTCGTA43900.033.32102238
GTATCAA155350.033.150961
ATCTCGT44000.033.08815837
GCTCGTC23200.033.0127739
GATCTCG44150.032.897636
TGCGACG17400.032.11898822
ATGCCGT48200.031.7089644
CGATAAT24100.030.92190267
TCGTCTA25200.030.66769454
Produced by FastQC (version 0.11.5)