FastQCFastQC Report
Sat 18 Jun 2016
SRR3137842_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137842_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2477469
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG136830.5522975262253533No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29000.042.1517561
GTATCAA61450.035.2463461
GCTCGAT4950.030.6841464
CAATGCG4600.027.74051519
TACCTGG15350.027.5913722
ATGCGAC4800.027.30371721
ATCAACG80450.026.1157953
TCAACGC81550.025.8965194
GTACTAG6950.025.7634411
CAACGCA83700.025.6486265
CTCGATA5800.025.59423465
TATCAAC82250.025.5789012
CGGGATA2700.025.56700550
TGGTCGT5750.025.21384651
GTCGTCT6150.024.6974953
AATGCGA5400.024.26947820
AACGCAG91300.023.7443566
CGCACTA4850.023.48474558
GTACCTG19000.023.1975291
CATTGCG6400.023.17880429
Produced by FastQC (version 0.11.5)