FastQCFastQC Report
Sat 18 Jun 2016
SRR3137842_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137842_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2477469
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31940.12892189569274126No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29250.042.951021
GCTCGAT4000.033.63709664
GTATCAA63650.033.3483771
TATGCCG8850.032.3568543
CAATGCG4900.030.27331419
TACCTGG14100.029.8507062
GTACTAG7450.028.7232381
CTCGATA4700.028.62731665
TAGTCGG3750.028.52023540
TCTCGTA9500.028.32597738
AACGACG500.00773782927.59911367
ATGCGAC5500.027.59911221
ATCGTCA5250.027.59799610
CTCGTAT9450.027.38062539
CGCACTA4550.027.29692658
TCGTATG10300.027.1312940
ATCTCGT10700.027.08433537
TTAGTCG3950.027.07562339
AATCTCG11050.026.85089936
TGGTCGT5200.025.87573251
Produced by FastQC (version 0.11.5)