FastQCFastQC Report
Sat 18 Jun 2016
SRR3137841_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137841_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5026739
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG400560.796858559793934No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55400.041.5274731
GTATCAA117350.033.251831
TGGTCGT8800.030.98635351
GCTCGAT9650.027.18366664
ATCAACG142700.026.8433133
TCAACGC144350.026.588184
CAACGCA147100.026.543535
CTCGATA9950.026.3651165
TATCAAC153100.025.1943382
GTCGTCT10700.024.83744853
AACGCAG158750.024.7305456
GCTCGTC11400.023.3095539
TCGATAA12200.021.78584166
CGATAAT12250.021.69778467
CGCAGAG180650.021.3746538
CGTACGA5050.021.1866260
TCGTCTA12950.020.78920654
ACGCAGA188250.020.6209837
GTACATG181500.020.3796371
AGAGTAC182200.020.32705911
Produced by FastQC (version 0.11.5)