FastQCFastQC Report
Sat 18 Jun 2016
SRR3137841_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137841_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5026739
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG115820.230407825033287RNA PCR Primer, Index 39 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG53140.10571465914581998RNA PCR Primer, Index 39 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58300.039.9596751
GTATCAA116850.032.874051
TATGCCG39150.031.98844143
CTCGTAT38750.031.24963839
TCTCGTA38250.031.2071638
TCGTATG42250.030.21265840
ATCTCGT40000.029.84214437
ATGCCGT42900.029.35308844
CCGTCTT43500.029.26603947
ATGCGAC10300.028.8046221
TGCCGTC44250.028.61378745
ATCGTCA10400.027.19997410
ATCAACG141500.026.5287383
TCAACGC142450.026.3757744
CAACGCA142650.026.2659765
TGGTCGT10350.026.00072351
CGCACTA10000.025.87520658
GCTCGAT10300.025.79146664
CGTATGC50200.025.6344141
CATCTCG46750.025.6071836
Produced by FastQC (version 0.11.5)