FastQCFastQC Report
Sat 18 Jun 2016
SRR3137840_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137840_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2998623
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG320711.0695242449617708No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36250.039.3283461
TACCTGG21350.037.0986252
GTATCAA71200.033.7589381
GTACCTG26050.031.8583031
ACCTGGG25850.029.9803663
ATCAACG87250.027.3578973
TCAACGC87750.027.2882694
CAACGCA89300.027.1704885
TAACGCA3050.025.9816274
TATCAAC91800.025.7716332
AACGCAG95350.025.6676756
GTTATCG1651.1459633E-1025.10227249
TATCACG2850.024.1664582
ATCACGC3100.022.2220063
CGCAGAG108250.022.1382738
CGTACGA1253.4946206E-622.0885260
ACGCAGA110150.021.9427647
GTACATG97700.021.3242681
ACATGGG91400.021.028243
ATAACGC4400.020.3533363
Produced by FastQC (version 0.11.5)