FastQCFastQC Report
Sat 18 Jun 2016
SRR3137840_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137840_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2998623
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG97260.3243488761341456RNA PCR Primer, Index 39 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG44430.14816800911618433RNA PCR Primer, Index 39 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38250.041.6852041
TACCTGG27900.035.740152
GTACCTG30050.033.8804321
TATGCCG33250.032.68422343
CTCGTAT32650.032.6508639
TCTCGTA32650.032.33386238
ACCTGGG31450.031.156313
ATGCCGT34900.031.04012944
CCGTCTT34950.030.79881347
TCGTATG35650.030.67743540
TGCCGTC35100.030.56890145
GTATCAA71650.030.4901051
ATCTCGT35300.030.10237
GCCGTCT35550.030.08490646
CGTCTTC38200.028.44944248
CATCTCG37650.028.03938736
TATCACG2500.027.6027742
CGTATGC40950.026.8754741
ATCACGC2900.024.9844383
CTACATC42650.024.8343733
Produced by FastQC (version 0.11.5)