FastQCFastQC Report
Sat 18 Jun 2016
SRR3137839_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137839_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1452355
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG203981.404477555418613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG1300.050.357772
ATAACGC1700.042.5653843
GGTATCA17350.042.2981531
TACCTGG12850.039.6839452
GTACCTG13950.036.800471
TAACGCA2050.035.3029984
ACCTGGG15300.032.205563
ATCACGC2150.032.053673
GTATCAA32800.031.5129221
TATACCG802.783605E-630.1577635
TCACGCA2600.027.8350544
GCCGCAC7550.027.4337566
GGTCGAC1555.2750693E-1126.6750832
CGCACCC7150.026.55446268
GGCCGCA7700.026.45100465
TCGACTC1608.0035534E-1125.8468364
GTCGACT1651.1641532E-1025.0601433
ATCAACG39850.024.9893953
TCAACGC40250.024.9157094
ATGGCCG8350.024.80193963
Produced by FastQC (version 0.11.5)