FastQCFastQC Report
Sat 18 Jun 2016
SRR3137839_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137839_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1452355
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG61420.4228993600049575TruSeq Adapter, Index 1 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG24700.17006861270144008TruSeq Adapter, Index 1 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGG16130.11106100092608212TruSeq Adapter, Index 1 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGC1850.048.4876753
TACCTGG16400.040.8119742
TAACGCA2300.039.0009544
CTCGTAT19450.037.24898539
TCTCGTA19250.036.91911338
GGTATCA15250.036.4338761
TATGCCG21450.036.34928543
GTACCTG18800.036.1624371
TCGTATG21500.036.10429440
ATGCCGT21800.035.76569744
ACCTGGG18050.035.5521163
TGCCGTC21950.035.52128645
GCCGTCT22350.035.1942846
CCGTCTT22700.034.65163847
CGTATGC22450.034.57649241
CGTCTTC22750.034.27218648
ATCTCGT21150.033.9287437
GATCTCG22000.032.30422636
GTATGCC24350.032.16189642
TATCACG1305.456968E-1231.8469312
Produced by FastQC (version 0.11.5)