FastQCFastQC Report
Sat 18 Jun 2016
SRR3137838_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137838_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4651395
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG263750.5670341908180234No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52200.039.1985321
GTATCAA106850.034.592271
ATCAACG132750.027.2254053
TCAACGC135000.026.8769154
CTCGATA9450.026.66425765
CAACGCA138650.026.5970135
GCTCGAT9550.026.38419764
GTGGTAT24250.025.4270021
TATCAAC147100.024.8438662
AACGCAG152200.024.3455146
CGCACTA8850.024.17570358
TGGTCGT10750.024.0788151
CGATAAT11150.022.91040467
ATGCGAC9700.022.75695621
GTCGTCT11800.021.93478453
CGCAGAG167350.021.6748228
CGTCATT12400.021.4217326
TCGTCTA12450.021.06701754
ATCGTCA10000.021.03566210
TCGATAA12050.020.91097366
Produced by FastQC (version 0.11.5)