FastQCFastQC Report
Sat 18 Jun 2016
SRR3137838_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137838_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4651395
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG71400.15350233639585545TruSeq Adapter, Index 1 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50900.039.463051
GTATCAA101150.033.779621
TATGCCG27250.032.28462243
CTCGTAT26450.031.56542839
ATCTCGT26150.031.53209937
TCTCGTA26500.031.11563938
TCGTATG28550.030.69372640
GATCTCG27200.030.31486936
ATGCCGT30800.028.67520944
CTCGATA10200.028.41226265
GTACTAG13350.028.179331
CCGTCTT31650.028.1234147
TGCCGTC31750.027.81750945
ATGCGAC10400.026.86950721
CGTATGC33000.026.65898341
ATCAACG126500.026.3461973
CAACGCA128100.026.044065
TCAACGC130500.025.670844
ATCGTCA10300.025.45401810
GCTCGAT11650.025.17211364
Produced by FastQC (version 0.11.5)