FastQCFastQC Report
Sat 18 Jun 2016
SRR3137837_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137837_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4114209
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG215210.5230896145528825No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48250.042.049231
GTATCAA101900.034.7841381
ATCAACG128600.027.4336513
TCAACGC130450.027.1277924
CAACGCA133300.027.042895
TATCAAC130350.026.901522
ATGCGAC6150.025.79878821
AACGCAG140450.025.7187166
AATGCGA6900.023.4947420
ACGCAGA157750.022.7527527
CAATGCG6850.022.65888619
TACCTGG22650.022.5078262
CGCAGAG160650.022.1711398
CGCACTA7000.021.69196558
GTACATG151550.021.206341
GTGGTAT25200.021.0503941
TCTAACG2800.020.9137022
AGAGTAC162200.020.85767711
TAGCGCG1507.342769E-720.69965729
CTCGATA5900.020.47524665
Produced by FastQC (version 0.11.5)