FastQCFastQC Report
Sat 18 Jun 2016
SRR3137837_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137837_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4114209
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG56330.1369157473526503RNA PCR Primer, Index 27 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49050.040.392721
GTATCAA101200.034.1756971
TATGCCG21450.030.39896443
CTCGTAT21400.029.50304839
TCTCGTA22000.028.6984238
TATCTCG23050.027.69013636
TCGTATG23700.027.65889540
ATCAACG127050.026.748643
ATGCCGT24650.026.73192244
TCAACGC127350.026.6314434
CTCGATA5700.026.63060465
CAACGCA128350.026.5314775
TATCAAC129400.026.4238032
CCGTCTT25100.025.42890247
AACGCAG138400.024.8535616
ATCTCGT26300.024.66189237
GCCGTCT26350.024.09166346
TGCCGTC26800.023.94460745
TACCTGG24050.023.3845212
GCTCGAT6650.022.30745364
Produced by FastQC (version 0.11.5)