FastQCFastQC Report
Sat 18 Jun 2016
SRR3137834_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137834_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4272095
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG258430.6049256863435856No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG58030.1358349943060723No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGC44640.10449205834608079No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52750.037.2127461
TGGTCGT14750.033.69439351
GTATCAA102450.032.9086461
GCTCGAT15700.031.66002764
CTCGATA15650.031.54061165
ATCGTCA14400.029.93313810
GTACTAG16900.029.7512681
ATGCGAC15300.028.85256821
CGACGAG15200.028.81955124
GTCGTCT17650.028.35608153
TCAGCGC18500.028.17532544
CGTCATT18700.027.48638526
CAATGCG16600.027.2172619
GCTCGTC19250.026.53276339
CAACGCA125950.026.295435
CGATAAT18800.026.25711667
TCGTCTA18800.026.25403254
ATCAACG125000.026.2069643
GTCGTCA20200.025.4426324
TCAACGC129100.025.3542374
Produced by FastQC (version 0.11.5)