FastQCFastQC Report
Sat 18 Jun 2016
SRR3137834_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137834_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4272095
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTCGTCTACCAGCTCGATAATTG71680.16778653096431612No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG65490.15329715280207953TruSeq Adapter, Index 11 (96% over 28bp)
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGC55430.1297489873235497No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51800.037.842621
ATCGTCA13850.035.86677610
GCTCGAT14350.035.1015564
TGGTCGT14750.034.8517651
CTCGATA14850.033.68656565
GTATCAA103000.033.238171
TATGCCG27600.033.00086243
CGACGAG15050.032.55082324
GTCGTCT16000.032.34497553
CTCGTAT27100.032.208239
TCTCGTA27150.031.64059838
GATCTCG27350.031.53499636
ATCTCGT27550.031.18084337
CAATGCG15750.031.10193419
TCAGCGC16900.030.8262544
TCGTATG29400.030.74462340
ATGCGAC16300.030.68919221
ATGCCGT31200.029.1930744
AATGCGA16950.029.10354820
ACGAGTC16950.028.69919226
Produced by FastQC (version 0.11.5)