FastQCFastQC Report
Sat 18 Jun 2016
SRR3137833_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3137833_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4277035
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG277570.648977621179158No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49200.040.3194961
GTATCAA97300.032.4574361
ATCAACG123500.025.4073853
CAACGCA127250.025.047565
GCTCGAT6900.025.01302364
TCAACGC126700.024.7980944
TATCAAC125850.024.7102412
CTCGATA7100.024.30985665
GTACTAG12350.023.4244821
AACGCAG140200.022.9840456
CCGATAA11750.022.02912938
GTGGTAT21900.021.8589061
CGTACGA3650.021.74747760
TCGATAA8450.021.65159666
CGATAAT8050.021.4407467
GTACATG151400.021.200581
ATGCGAC7400.020.9739221
ACCGATA12350.020.95618237
TACCGAT12700.020.37984736
ACATGGG148150.020.3662473
Produced by FastQC (version 0.11.5)